Phospho-eNOS (Thr495) Polyclonal Antibody, HRP Conjugated
Product Specifications
Background
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Synonyms
ENOS; ECNOS; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; NOS3
Gene ID
4846
Swiss Prot
P29474
Modification Site
Thr495
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Cross Reactivity
Human, Mouse
Target
Phospho-eNOS (Thr495)
Clonality
Polyclonal
Conjugation
HRP
Source
KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Thr495
Applications
WB, ELISA, IHC-P, IHC-F
Purification
Purified by Protein A.
Concentration
1ug/ul
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Prediction Reactivity
Rat, Dog, Cow, Sheep, Pig, Rabbit, GuineaPig
Gene ID URL
4846
Isotype
IgG
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC740257-100 1x 100 µL
Cytokeratin, HMW (AE-3), CF740 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC740218-100 1x 100 µL
CD100 (Semaphorin-4D) (A8), CF740 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC040745-100 1x 100 µL
CD45 / LCA (2B11 + PD7/26), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC740012-500 1x 500 µL
Tyrosinase (T311), CF740 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC940391-100 1x 100 µL
CD81 / TAPA-1 (1.3.3.22), CF594 conjugate, 0.1mg/mL
Sign In for Pricing
View Details