Phospho-eNOS (Thr495) Polyclonal Antibody, PE Conjugated

Product Specifications

Background

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Synonyms

ENOS; ECNOS; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; NOS3

Gene ID

4846

Swiss Prot

P29474

Modification Site

Thr495

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

Phospho-eNOS (Thr495)

Clonality

Polyclonal

Conjugation

Source

KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Thr495

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

496,564nm/578nm

Concentration

1ug/ul

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Prediction Reactivity

Rat, Dog, Cow, Sheep, Pig, Rabbit, GuineaPig

Gene ID URL

4846

Isotype

IgG

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

SC112626 10 µg

Pumilio 1 (PUM1) (NM_014676) Human Untagged Clone

Ask

View Details

REC31989-100 1 Each

Norovirus GII.17 VLP

Ask

View Details

SYP-H0459-01 48 Well

Human PAP(Plasmin-Antiplasmin Complex) ELISA Kit

Ask

View Details

SYP-H0459-02 96 Well

Human PAP(Plasmin-Antiplasmin Complex) ELISA Kit

Ask

View Details

NBP3-05975MFV610 0.1 mL

Rabbit Polyclonal CFAP161 Antibody [mFluor Violet 610 SE]

Ask

View Details