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Phospho-c-Jun (Ser63) Polyclonal Antibody

Product Specifications

Background

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.

Synonyms

AP1; AP-1; c-Jun; Transcription factor AP-1; Activator protein 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUN

Gene ID

3725

Swiss Prot

P05412

Modification Site

Ser63

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-c-Jun (Ser63)

Clonality

Polyclonal

Conjugation

Unconjugated

Source

KLH conjugated synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Ser63

Applications

WB, ELISA, FCM, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1ug/ul

Dilution

WB (1:300-5000), ELISA (1:500-1000), FCM (1:20-100), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Prediction Reactivity

Dog, Cow, Sheep, Pig, Rabbit

Gene ID URL

3725

Isotype

IgG

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