Phospho-H2AX (Ser139) Polyclonal Antibody, AbBy Fluor™ 488 Conjugated

Product Specifications

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Synonyms

H2AX; H2A.X; H2A/X; Histone H2AX; Histone H2A.X; H2AFX

Gene ID

3014

Swiss Prot

P16104

Modification Site

Ser140

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-H2AX (Ser139)

Clonality

Polyclonal

Conjugation

AbBy Fluor™ 488

Source

KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

499nm/519nm

Concentration

1ug/ul

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

This phosphorylation site is homologous to that of Ser140 in Mouse and Rat.

Prediction Reactivity

Dog, Cow, Pig, Horse, Rabbit

Gene ID URL

3014

Isotype

IgG

Curated Selection

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