Phospho-FLT3 (Tyr842) Polyclonal Antibody
Product Specifications
Background
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
Synonyms
FLK2; STK1; CD135; FLK-2; Receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; Fetal liver kinase-2; Fms-like tyrosine kinase 3; FLT-3; Stem cell tyrosine kinase 1; STK-1; FLT3
Gene ID
2322
Swiss Prot
P36888
Modification Site
Tyr842
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Cross Reactivity
Rat
Target
Phospho-FLT3 (Tyr842)
Clonality
Polyclonal
Conjugation
Unconjugated
Source
KLH conjugated synthetic phosphopeptide derived from human FLT3 around the phosphorylation site of Tyr842
Applications
WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1ug/ul
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Prediction Reactivity
Human, Mouse, Rabbit
Gene ID URL
2322
Isotype
IgG
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
