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E.coli DH-5 Alpha Polyclonal Antibody, RBITC Conjugated

Product Specifications

Background

This antibody reacts with Escherichia coli HD5a (E.coli HD5a), Escherichia coli is a gram-negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. E. coli DH5_ strain is an engineering strain widely used in biopharmaceuticals. According to the requirements of product quality inspection, it is necessary to detect the residual amount of DH5_ bacterial protein in biological products. This product is an anti-serum obtained by immunizing rabbits with DH5_ bacterial protein as an antigen, and purified by affinity chromatography to obtain antibody IgG, which can be used for ELISA, Western Blot and other experimental tests.

Synonyms

Escherichia coli DH-5 Alpha; E.coli DH5 Alpha; E.coli DH 5 Alpha.

Host

Rabbit

Cross Reactivity

Bacteria, Others

Target

E.coli DH-5 Alpha

Clonality

Polyclonal

Conjugation

RBITC

Source

Whole cell protein of Ecoli DH-5 Alpha

Applications

WB, IF (IHC-P)

Purification

Purified by Protein A.

Excitation Emission

570nm/595nm

Concentration

1ug/ul

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Isotype

IgG

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