Fx1A Polyclonal Antibody, Cy3 Conjugated
Product Specifications
Background
Binding of anti-Fx1A to Heymann nephritis antigens (HA) on rat glomerular epithelial cells (GECs) in culture leads to capping and disappearance of antigens from the cell surface. This process may contribute to the formation of glomerular subepithelial immune deposits in vivo. The authors differentially extracted GECs to determine whether HA redistribution is mediated by cytoskeletal components. Observations were made by phase-contrast and immunofluorescence microscopy on primary and passaged GECs in monolayer culture and by spectrofluorimetry on GECs in suspension.
Cellular Locus
Cytoplasm
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Fx1A
Clonality
Polyclonal
Conjugation
Cy3
Source
Fx1A protein
Applications
IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
512,550nm/570,615nm
Concentration
1ug/ul
Dilution
IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Isotype
IgG
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS6400195-01 0.1 mL
Complement Component C8 (Complement C8, Complement Component C8 alpha Chain, Complement Component 8 alpha Polypeptide, Complement Component 8 Subunit alpha, C8A, Complement Component C8 beta Chain, Complement Component 8 beta Polypeptide, Complement Compo
Ask
View Details 
MBS6400195-02 5x 0.1 mL
Complement Component C8 (Complement C8, Complement Component C8 alpha Chain, Complement Component 8 alpha Polypeptide, Complement Component 8 Subunit alpha, C8A, Complement Component C8 beta Chain, Complement Component 8 beta Polypeptide, Complement Compo
Ask
View Details 
