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Anti-Calpastatin Antibody

Mouse Monoclonal Antibody specific to Human Calpastatin

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Calpain inhibitor, Sperm BS-17 component

Gene Name

CAST

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=CAST">CAST</a>

UniProt

P20810

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human muscle- type calpastatin.

Target Antigen

Calpastatin

Target

Calpastatin

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA

Field of Research

Neuroscience

Purification Method

Purified by immunoaffinity chromatography

Dilution

Reconstitute with sterile H2O. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Format

Purified

Form

Lyophilized

Buffer

Phosphate Buffered Saline

Function

Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.

Additionnal Information

ELISA: 1-10ug/ml with immobilized antigen <br><br>Western Blot: 1-10ug/ml, denaturing and non-denaturing conditions. <br><br>Immunohistochemistry: 1-10ug/ml, frozen tissue sections.

Storage Conditions

This product does not contain preservative. Lyophilized antibody is stable at 4°C for 2 years. Store the reconstituted solution (2. 0mg/ml) in aliquots at -20°C for one year or at 4°C for 6 months after adding 0.1% sodium azide. Dilutions of the stock solution should not be stored. Avoid repeated freeze-thaw cycles.

Specificity

This antibody specifically recognizes an epitope within domain III of human calpastatin.

Formulation

10 mM PBS, pH 7.4, 1% BSA, lyophilized

Buffer pH

pH 7.4

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