Phospho-eNOS (Ser632) Polyclonal Antibody, AbBy Fluor™ 594 Conjugated

Product Specifications

Background

ENOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.

Synonyms

ENOS phospho S633; p-eNOS phospho S633; eNOS 633; cNOS; eNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN.

Gene ID

4846

Modification Site

Ser633

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Target

Phospho-eNOS (Ser632)

Clonality

Polyclonal

Conjugation

AbBy Fluor™ 594

Source

KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Ser633

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

590nm/617nm

Concentration

1ug/ul

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Prediction Reactivity

Human, Mouse, Rat, Dog, Sheep, Pig, Rabbit

Gene ID URL

4846

Isotype

IgG

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