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AGEs Polyclonal Antibody, Cy5 Conjugated

Product Specifications

Background

Advanced Glycation End products (AGEs) are the result of a chain of chemical reactions after an initial glycation reaction. The intermediate products are known, variously, as Amadori, Schiff base and Maillard products, named after the researchers who first described them. (The literature is inconsistent in applying these terms. For example, Maillard reaction products are sometimes considered intermediates and sometimes end products.) Side products generated in intermediate steps may be oxidizing agents (such as hydrogen peroxide), or not (such as beta amyloid proteins) . "Glycosylation" is sometimes used for "glycation" in the literature, usually as 'non-enzymatic glycosylation. The AGE-modified BSA was produced by reacting BSA with glycolaldehyde under sterile conditions followed by extensive dialysis and purification steps.

Synonyms

Advanced Glycation End products; Serum albumin; BSA; ALB

Gene ID

280717

Swiss Prot

P02769

Cellular Locus

Secreted

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

AGEs

Clonality

Polyclonal

Conjugation

Cy5

Source

AGEs

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

625,650nm/670nm

Concentration

1ug/ul

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

Reacts with AGEs.

Gene ID URL

280717

Isotype

IgG

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