CHAPSO

CHAPSO is a bile derivative. CHAPSO reduces protein adsorption, improves the cryo-EM imaging quality. CHAPSO can be used as a detergent, or as the membrane protein solubilization and reconstruction reagent in membrane protein structural studies[1][2][3].

Product Specifications

CAS Number

[82473-24-3]

Product Name Alternative

3-[3- (Cholamidopropyl) dimethylammonio]-2-hydroxy-1-propanesulfonate

UNSPSC

12352200

Hazard Statement

H302, H315, H319, H335

Target

Biochemical Assay Reagents

Type

Biochemical Assay Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/chapso.html

Purity

99.92

Solubility

DMSO : 100 mg/mL (ultrasonic) |H2O : 100 mg/mL (ultrasonic)

Smiles

O[C@H]1[C@@]2([H])[C@@]3([H])[C@@]([C@@](CC3)([H])[C@H](C)CCC(NCCC[N+](C)(C)CC(O)CS(=O)([O-])=O)=O)([C@H](C[C@]2([H])[C@@]4([C@](C[C@@H](CC4)O)([H])C1)C)O)C

Molecular Formula

C32H58N2O8S

Molecular Weight

630.88

Precautions

H302, H315, H319, H335

References & Citations

[1]Li M, et al. Morphological characterization of DMPC/CHAPSO bicellar mixtures: a combined SANS and NMR study. Langmuir. 2013 Dec 23;29 (51) :15943-57.|[2]Chen J, et al. Eliminating effects of particle adsorption to the air/water interface in single-particle cryo-electron microscopy: Bacterial RNA polymerase and CHAPSO. J Struct Biol X. 2019 Jan-Mar;1:100005.|[3]Cladera J, et al., Liposome solubilization and membrane protein reconstitution using Chaps and Chapso. Eur J Biochem. 1997 Feb 1;243 (3) :798-804.