Phospho-AKT (Ser473) Polyclonal Antibody, Biotin Conjugated
Product Specifications
Background
AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
Synonyms
Akt; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha; Akt1
Gene ID
24185
Swiss Prot
P47196
Modification Site
Ser473
Cellular Locus
Cytoplasm, Nucleus, Cell membrane
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-AKT (Ser473)
Clonality
Polyclonal
Conjugation
Biotin
Source
KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473
Applications
WB, IHC-P, IHC-F
Purification
Purified by Protein A.
Concentration
1ug/ul
Dilution
WB (1:500-2000), IHC-P (1:100-500), IHC-F (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C for 12 months.
Gene ID URL
24185
Isotype
IgG
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC040029-500 1x 500 µL
Fibronectin (TV-1), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC401073-500 1x 500 µL
HLA-Aw32 & HLA-A25 (MHC I) (CATA-1), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC400040-100 1x 100 µL
CD35 / CR1 (E11), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC941081-500 1x 500 µL
CD45RO (190-2F2.5), CF594 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC040633-100 1x 100 µL
Von Willebrand Factor (3E2D10), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC040021-100 1x 100 µL
Cytokeratin 18 (DC10), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details