Phospho-AKT (Ser473) Polyclonal Antibody, Cy5 Conjugated
Product Specifications
Background
AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
Synonyms
Akt; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha; Akt1
Gene ID
24185
Swiss Prot
P47196
Modification Site
Ser473
Cellular Locus
Cytoplasm, Nucleus, Cell membrane
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-AKT (Ser473)
Clonality
Polyclonal
Conjugation
Cy5
Source
KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473
Applications
WB, IF
Purification
Purified by Protein A.
Excitation Emission
625,650nm/670nm
Concentration
1ug/ul
Dilution
WB (1:500-2000), IF (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
24185
Isotype
IgG
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