Tyrosinase Polyclonal Antibody
Product Specifications
Background
Tyrosinase is the key enzyme for melanin, synthesis in mammalian melanocytes and has been considered to be a unique marker for the study of melanocyte differentiation. A cDNA library was constructed from poly (A) + mRNA from mouse melanocytes and screened using anti- tyrosinase antiserum and oligonucleotide probes corresponding to amino acid sequence of tyrosinase. sequencing of some cDNA clones positive in these screenings gave a nucleotide sequence of 1838 nucleotides including a open reading frame of 1344 nucleotides that was found to correspond exactly to the amino acid sequence of the cyanogen bromide fragments of tyrosinase.
Synonyms
LB24 AB; LB24-AB; Monophenol monooxygenase; SK29 AB; Tumor rejection antigen AB; LB24 AB; Monophenol monooxygenase; OCA1A; OCAIA; Oculocutaneous albinism IA; SK29 AB; SK29-AB; Tumor rejection antigen AB; TYR; tyrosinase oculocutaneous albinism IA; TYRO_HUMAN.
Gene ID
7299
Cellular Locus
Cytoplasm
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Immunogen
155-250/529
Target
Tyrosinase
Clonality
Polyclonal
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human Tyrosinase
Applications
WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1ug/ul
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Prediction Reactivity
Dog, Cow, Sheep, Pig
Gene ID URL
7299
Isotype
IgG
Curated Selection
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