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F (ab') ₂ Fragment Goat Anti-Mouse IgG (H+L)

Product Specifications

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Host

Goat

Cross Reactivity

Mouse

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Applications

FCM, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein G.

Concentration

Lyophilized

Dilution

FCM (1:20-100), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M PBS (pH7.4)

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

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