Recombinant AmCyan-Tag protein, His
Product Specifications
Background
AmCyan1 is a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (AmCyan) that exhibits enhanced emission characteristics (excitation and emission maxima: 458nm and 489nm, respectively) . DD-AmCyan1 is a modified version of AmCyan1 that is tagged on its N-terminus with the ProteoTuner destabilization domain (DD) . The presence of this destabilization domain causes rapid, proteasomal degradation of the fluorescent fusion protein; however, when the membrane permeant ligand Shield1 is added to the medium, it binds to the destabilization domain and protects the fusion protein from degradation.
Expression System
E.coli
Conjugation
Unconjugated
Tag
His
Source
Recombinant AmCyan-Tag protein, His protein is expressed in E.coli with His. It contains the amino acid sequence of 1-234/234.
Applications
ELISA
Endotoxin
Not Tested
Purity
> 95% as determined by SDS- PAGE
Buffer
20mM Tris-Hcl (pH=9.0) with 8M Urea and 1mM DTT.
Storage Conditions
The product should be stored at -70°C or -20°C.
Species
Others
Available Sizes
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