Phospho-Nucleolin (Thr76) Recombinant Antibody, HRP Conjugated
Product Specifications
Background
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Synonyms
MS1116; NCL; Nucl; Nucleolin; Protein C23.
Gene ID
4691
Swiss Prot
P19338
Modification Site
Thr76
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human
Target
Nucleolin
Clonality
Recombinant
Isotype
IgG
Conjugation
HRP
Source
KLH conjugated synthetic peptide derived from human NCL around the phosphorylation site of T76
Applications
WB, IHC-P, IHC-F
Purification
Purified by Protein A.
Concentration
Lot dependent
Dilution
WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
4691
Curated Selection
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