Phospho-Nucleolin (Thr76) Recombinant Antibody, Biotin Conjugated

Product Specifications

Background

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Synonyms

MS1116; NCL; Nucl; Nucleolin; Protein C23.

Gene ID

4691

Swiss Prot

P19338

Modification Site

Thr76

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human

Target

Nucleolin

Clonality

Recombinant

Isotype

IgG

Conjugation

Biotin

Source

KLH conjugated synthetic peptide derived from human NCL around the phosphorylation site of T76

Applications

WB, IHC-P, IHC-F

Purification

Purified by Protein A.

Concentration

Lot dependent

Dilution

WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C for 12 months.

Gene ID URL

4691

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