UBE2I Recombinant Antibody
Product Specifications
Background
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity.
Synonyms
C358B7.1; P18; UBC9; UBE2I; SUMO1 protein ligase.
Gene ID
7329
Swiss Prot
P63279
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse
Target
UBE2I
Clonality
Recombinant
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human UBE2I
Applications
WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF
Purification
Purified by Protein A.
Concentration
Lot dependent
Dilution
WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500), IP (1-2ug), IF (), Flow-Cyt (1ug/Test), ICC/IF (1:50-100)
Buffer
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
7329
Predicted Cross Reactivity
Rat
Available Sizes
Curated Selection
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