Histone H2A.X Recombinant Antibody, Biotin Conjugated

Product Specifications

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Synonyms

H2AX_HUMAN; 2A.X variant histone; H2A.X; H2AX; H2A/X; H2AFX; Histone H2AX

Gene ID

3014

Swiss Prot

P16104

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Histone H2A.X

Clonality

Recombinant

Isotype

IgG

Conjugation

Biotin

Source

KLH conjugated synthetic peptide derived from human H2AX

Applications

WB, IHC-P, IHC-F

Purification

Purified by Protein A.

Concentration

Lot dependent

Dilution

WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C for 12 months.

Product Datasheet

https://www.biossantibodies.com/datasheets/bsm-61080R-Biotin

Gene ID URL