Phospho-Histone H3 (Ser10) monoclonal Antibody
Product Specifications
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Synonyms
Histone H3 (phospho S10) ; Phospho-Histone H3 (Ser10) ; Histone H3 (Phospho-Ser10) ; Histone H3 (p-Ser10) ; Histone H3 (Phospho-S10) ; H3S10ph; PHH3; H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; H31_HUMAN; Histone H3; H3S; Histone H3-I/H3-II; Major histone H3; H3F; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l.
Gene ID
8350
Swiss Prot
P68431
Cellular Locus
Nucleus
Host
Mouse
Cross Reactivity
Human
Target
Phospho-Histone H3 (Ser10)
Clonality
Monoclonal
Isotype
IgG
Clone
A1F11
Conjugation
Unconjugated
Source
KLH conjugated synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser10
Applications
WB, IHC-P, IHC-F, IF
Purification
Purified by Protein G.
Concentration
1mg/ml
Dilution
WB (WB=1:1000-2000), IHC-P (IHC-P=1:100-500), IHC-F (IHC-F=1:400-800), IF (IF=1:100-500)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
8350
Predicted Cross Reactivity
Mouse, Rat, Cow, Pig, Rabbit, Firefly
Curated Selection
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