O-GlcNAcase (1A5) Monoclonal Antibody
Product Specifications
Background
Isoform 1: - Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) . Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Isoform 3: - Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
Synonyms
Protein O-GlcNAcase; Beta-N-acetylglucosaminidase; Beta-N-acetylhexosaminidase; Beta-hexosaminidase; Meningioma-expressed antigen 5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; OGA; NCOAT; MGEA5; HEXC; KIAA0679; MEA5
Gene ID
10724
Swiss Prot
O60502
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse
Target
OGA
Clonality
Monoclonal
Isotype
IgG
Clone
1A5
Conjugation
Unconjugated
Source
Recombinant protein of N-terminal human MGEA5.
Applications
WB, IHC-P, IP
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), IHC-P (1:200-400), IP (1-2ug)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C for 12 months.
Gene ID URL
10724
Curated Selection
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