BUB3 Recombinant Antibody
Product Specifications
Background
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Synonyms
Mitotic checkpoint protein BUB3; BUB3
Gene ID
9184
Swiss Prot
O43684
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Bub3
Clonality
Recombinant
Isotype
IgG
Conjugation
Unconjugated
Source
Recombinant protein corresponding to the N-terminus of human Bub3.
Applications
WB, FCM, IHC-P, IF (IHC-P), IF (ICC), IP
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IHC-P (1:200-400), IF (IHC-P) (1:50-200), IF (ICC) (1:50-200), IP (1-2ug)
Buffer
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
9184
Available Sizes
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