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MCM5 (9G7) Monoclonal Antibody

Product Specifications

Background

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Interacts with MCMBP.

Synonyms

DNA replication licensing factor MCM5; P1-CDC46; MCM5; CDC46; minichromosome maintenance complex component 5.

Gene ID

4174

Swiss Prot

P33992

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

MCM5

Clonality

Monoclonal

Isotype

IgG

Clone

9G7

Conjugation

Unconjugated

Source

Recombinant protein within C terminal human MCM5.

Applications

WB, IHC-P

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

WB (1:300-5000), IHC-P (1:200-400)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Gene ID URL

4174

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