DNA Ligase IV Recombinant Antibody
Product Specifications
Background
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V (D) J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Synonyms
DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4.
Gene ID
3981
Swiss Prot
P49917
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human
Target
LIG4
Clonality
Recombinant
Isotype
IgG
Conjugation
Unconjugated
Source
Recombinant protein within human DNA Ligase IV aa 500-700
Applications
WB, IHC-P, IHC-F, IF, ICC/IF
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500), IF (), ICC/IF (1:50-100)
Buffer
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
3981
Available Sizes
Curated Selection
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