H3K4me1 (34A1) Monoclonal Antibody
Product Specifications
Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Gene ID
8350
Swiss Prot
P68431
Host
Mouse
Cross Reactivity
Human
Target
H3K4me1
Clonality
Monoclonal
Isotype
IgG2b
Clone
34A1
Conjugation
Unconjugated
Source
Monoclonal antibody raised in mouse against histone H3, monomethylated at lysine 4 (H3K4me1), using a KLH-conjugated synthetic peptide
Applications
WB, ELISA, IHC-P, IF (ICC)
Purification
Protein A purified monoclonal antibody in PBS containing 0.05% azide.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C for 12 months.
Gene ID URL
8350
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