Ubiquitin-like modifier-activating enzyme 1 (8A4) Monoclonal Antibody
Product Specifications
Background
Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181) . Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181) . Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334) .
Synonyms
Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1, UBA1
Gene ID
7317
Swiss Prot
P22314
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Ubiquitin-like modifier-activating enzyme 1
Clonality
Monoclonal
Isotype
IgG
Clone
8A4
Conjugation
Unconjugated
Source
Human Ubiquitin-like modifier-activating enzyme 1 between 1000 amino acids to C-terminus
Applications
WB, FCM, IHC-P, IF (ICC), IHC
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IHC-P (1:200-400), IF (ICC) (1:50-200), IHC ()
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C for 12 months.
Gene ID URL
7317
Curated Selection
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