Ki67 Recombinant Antibody
Product Specifications
Background
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase) . It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index) . In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
Synonyms
Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN.
Gene ID
4288
Swiss Prot
P46013
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Ki67
Clonality
Recombinant
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human Ki67
Applications
WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500), IF (), Flow-Cyt (1ug/Test), ICC/IF (1:50-100)
Buffer
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
4288
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