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Phospho-Histone H3 (S10) Recombinant Antibody

Product Specifications

Background

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Synonyms

H3 3 like sequence MH921; H3 3A; H3 a; H3 b; H3 c; H3 d; H3 f; H3 h; H3 histone family member E pseudogene; H3 i; H3 j; H3 k; H3 l; H33_HUMAN; H3F3; H3f3b; Histone H3 3 pseudogene; Histone H3.3

Swiss Prot

P68431; P84243

Modification Site

Ser10

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-Histone H3 (S10)

Clonality

Monoclonal

Isotype

IgG

Clone

2F6

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding surrounding Ser10 of human Histone H3

Applications

IHC-P, IHC-F, IF

Purification

Purified by Protein A.

Concentration

Lot Dependent

Dilution

IHC-P (1:200-400), IHC-F (1:100-500), IF ()

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C for 12 months.

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