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Histone H3 (mono methyl K18) Recombinant Antibody, HRP Conjugated

Product Specifications

Background

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Synonyms

Histone H3, Histone H3

Swiss Prot

P68431

Modification Site

Mono methyl K18

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Histone H3 (mono methyl K18)

Clonality

Monoclonal

Isotype

IgG

Clone

2H4

Conjugation

HRP

Source

Synthetic peptide surrounding K18 of Histone H3

Applications

WB, IHC-P, IHC-F

Purification

Purified by Protein A.

Concentration

Lot dependent

Dilution

WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Methylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

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