Phospho-eIF4E (Ser209) Recombinant Antibody
Product Specifications
Background
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Synonyms
CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
Gene ID
1977
Swiss Prot
P06730
Modification Site
S209
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-eIF4E (Ser209)
Clonality
Recombinant
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated Synthesised phosphopeptide derived from human EIF4E around the phosphorylation site of Ser209: SG (p-S) TT
Applications
WB, IHC-P, IHC-F, IF, ICC/IF
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500), IF (), ICC/IF (1:50-100)
Buffer
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Modification
Phosphorylation
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
1977
Available Sizes
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