Cdk4 (2F6) Monoclonal Antibody
Product Specifications
Background
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G (1) /S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Synonyms
Cyclin-dependent kinase 4; CDK4; Cell division protein kinase 4; PSK-J3
Gene ID
1019
Swiss Prot
P11802
Host
Mouse
Cross Reactivity
Human
Target
Cdk4
Clonality
Monoclonal
Isotype
IgG2c
Clone
2F6
Conjugation
Unconjugated
Source
Recombinant human Cdk4 protein, around C-terminal 101aa.
Applications
WB, IHC-P, IHC-F, IF, ICC/IF
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (WB=1:500-2000), IHC-P (IHC-P=1:50-200), IHC-F (IHC-F=1:50-200), IF (IF=1:50-200), ICC/IF (ICC/IF=1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
1019
Curated Selection
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