SUFU Monoclonal Antibody
Product Specifications
Background
Negative regulator in the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein. Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL) . GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A) .
Synonyms
SU FU; SU (F) U; Su (fu) ; SUFU_HUMAN; SUFUH; SUFUXL; Suppressor of fused homolog (Drosophila) ; Suppressor of fused homolog; OTTHUMP00000020374; OTTHUMP00000020377; OTTHUMP00000020379; PRO1280.
Gene ID
51684
Swiss Prot
Q9UMX1
Cellular Locus
Cytoplasm, Nucleus
Host
Mouse
Target
SUFU
Clonality
Monoclonal
Clone
D6S12
Conjugation
Unconjugated
Source
Recombinant human SUFU.
Applications
WB, IHC-P, IHC-F, IF
Concentration
0.5µg/µl
Dilution
WB (WB=1:500-2000), IHC-P (IHC-P=1:100-500), IHC-F (IHC-F=1:100-500), IF (IF=1:100-500)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
51684
Predicted Cross Reactivity
Human, Mouse, Monkey
Curated Selection
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