EZH2 Monoclonal Antibody
Product Specifications
Background
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
Synonyms
Enhancer of zeste homolog 2; Enx1h; MGC9169; Enhancer of zeste 2; ENX-1; ENX 1; ENX1; EZH 2; EZH2_HUMAN; Histone-lysine N-methyltransferase EZH2; KMT6A; Lysine N-methyltransferase 6; Enhancer of zeste homolog 2 (Drosophila) ; Enhancer of zeste, Drosophila, homolog 2; KMT 6; KMT6A; KMT6; WVS2.
Gene ID
2146
Swiss Prot
Q15910
Cellular Locus
Nucleus
Host
Mouse
Cross Reactivity
Human
Target
EZH2
Clonality
Monoclonal
Clone
E3H5
Conjugation
Unconjugated
Source
Recombinant human EZH2.
Applications
WB, Flow-Cyt, ICC/IF
Concentration
0.5µg/µl
Dilution
WB (WB (1:500-2000) ), Flow-Cyt (Flow-Cyt (1ug/Test) ), ICC/IF (ICC/IF (1:50-200) )
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
2146
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