MGMT (4C12) Monoclonal Antibody
Product Specifications
Background
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Synonyms
Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
Gene ID
4255
Swiss Prot
P16455
Host
Mouse
Cross Reactivity
Human
Target
MGMT
Clonality
Monoclonal
Isotype
IgG1
Clone
4C12
Conjugation
Unconjugated
Source
Purified His-tagged MGMT protein was used to produced this monoclonal antibody.
Applications
WB, IHC-P, IHC-F, IF, ICC/IF
Purification
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Concentration
0.5µg/µl
Dilution
WB (WB=1:500-2000), IHC-P (IHC-P=1:100-500), IHC-F (IHC-F=1:20-100), IF (IF=1:20-100), ICC/IF (ICC/IF=1:100-500)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
4255
Curated Selection
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