H2AFX (9D5) Monoclonal Antibody

Product Specifications

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Synonyms

H2AX; H2A.X; H2A/X; Histone H2AX; Histone H2A.X; H2AFX

Gene ID

3014

Swiss Prot

P16104

Host

Mouse

Cross Reactivity

Human

Target

H2AFX

Clonality

Monoclonal

Isotype

IgG1

Clone

9D5

Conjugation

Unconjugated

Source

This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human H2AFX.

Applications

WB, IHC-P

Purification

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.