Phosphotyrosine Monoclonal Antibody, HRP Conjugated
Product Specifications
Background
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Synonyms
Phospho-tyrosine; Phospho tyrosine; Phospho-tyr; Phospho tyr; p-Tyr; p-tyrosine
Modification Site
Phosphoserine
Cellular Locus
Cytoplasm, Nucleus, Cell membrane
Host
Mouse
Cross Reactivity
Human, Mouse, Rat
Target
Phosphotyrosine
Clonality
Monoclonal
Isotype
IgG
Clone
4D3
Conjugation
HRP
Source
KLH conjugated Phosphotyrosine
Applications
IHC-P, IHC-F
Purification
Purified by Protein G.
Concentration
1µg/µl
Dilution
IHC-P (1:200-400), IHC-F (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
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