Mouse IgG2b Isotype Control PE Antibody
Mouse monoclonal antibody conjugated to PE which recognizes IgG2b that belongs to many immunoglobulin G. It can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG2b fluorochrome-conjugated antibodies to human cells. It was found that IgG2a was superior to IgG1 in activating complement. IgG2 is an antibody moleculer induced by infection.
Product Specifications
Immunogen
KLH-coupled trinitrophenol
Clonality
Monoclonal
Clone
MPC-11
Conjugation
PE
Purification
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Concentration
0.1 mg/ml
Storage Conditions
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Notes
For research use only.
Applications Notes
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Tested Applications
FC
Preservative
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Isotype
Mouse IgG2b kappa
Entrez
35
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