SAG

Smoothened agonist which bind directly to SMO and can block SMO inhibition by cyclopamine. The conformation of SMO is altered by SAG binding which results in the accumulation of SMO in cilia and activated gene transcription. It should be noted that SAG inhibits hedgehog signaling at concentrations greater than 1 µM (EC50 for SMO activation is ~ 3 nM) .1