Biotinyl-Tyramide

Reagent for use in catalyzed reporter deposition (CARD) signal amplification protocols in a variety of immunoassays in which, horseradish peroxidase catalyzed deposition of biotinyl tyramide is detected with labeled streptavidin.1 Widely used for signal amplification in fluorescent in situ hybridization (FISH) protocols.2 May be used for signal amplification in a sandwich ELISA for quantification of α-tubulin isotypes.3 Reagent for “self-biotinylation” of DNA G-quadruplexes.4 May be used with APEX-mediated biotin labeling to identify protein-protein interactions.5