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CD18 Antibody

Mouse monoclonal antibody to CD18 which belongs to the integrin beta chain family. Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) . CD18 is involved in cellular adhesion and the immune response. CD18 and the a subunits are expressed broadly by all leukocytes with NK and T cells showing higher density of surface expression.

Product Specifications

Product Name Alternative

Integrin beta2, Complement receptor C3 subunit beta, ITGB2

UniProt

P05107

Reactivity

Human

Immunogen

Peripheral blood mononuclear cells

Target

CD18

Clonality

Monoclonal

Clone

MEM-148

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Functional application: The antibody MEM-148 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes. Flow cytometry: Recommended dilution: 1-5 μg/ml. The antibody MEM-148 is an excellent marker of activated myeloid cells. Western blotting: Recommended dilution: 1-2 μg/ml.

Tested Applications

FC, Functional Studies, IP, WB

Preservative

Phosphate buffered saline (PBS), pH 7.4

Isotype

Mouse IgG1

Entrez

3689

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