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CD271 Antibody

Mouse monoclonal antibody to CD271 which is low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It can mediate cell survival as well as cell death of neural cells. CD271 is useful for the detection of all cells expressing p75NTR on their surface, such as motor neurons cholinergic basal forebrain neurons, activated Schwann cells, olfactory ensheathing glial cells.

Product Specifications

Product Name Alternative

TNFRSF16, NGFR, p75NTR, p75NGFR

UniProt

P08138

Reactivity

Feline, Ferret, Human, Primate, Rabbit

Immunogen

Purified CD271 protein isolated from human melanoma cell line A875

Target

CD271

Clonality

Monoclonal

Clone

NGFR5

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation; Cell Biology

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 1-2 μg/ml; Positive tissue: melanoma, heat-mediated antigen retrieval. Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 μg/ml.

Tested Applications

FC, ICC, IHC-P, IP, WB

Preservative

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Isotype

Mouse IgG1

Entrez

4804

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