Human Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer Protein, His Tag&Tag Free

Integrin alpha 2 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 subunit. It is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A and Human echoviruses 1 and 8. DGEA inhibited rotavirus binding to alpha2beta1 and infectivity. In a novel process, integrin-using viruses bind the alpha2 I domain of alpha2beta1 via DGE in VP4 and interact with alphaXbeta2 (via GPR) and alphaVbeta3 by using VP7 to facilitate cell entry and infection.

Product Specifications

Background

Specifications

Human ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA2 and ITGB1, has a calculated MW of 127.6 kDa (ITGA2) and 83.7 kDa (ITGB1) . Subunit ITGA2 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 150-180 kDa (ITGA2) and 100-120 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.

Accession Number

P17301-1 (E534K) (ITGA2) & P05556-1 (ITGB1)

Expression Region

Tyr 30 - Thr 1132 (ITGA2) & Gln 21 - Asp 728 (ITGB1)

Host

HEK293

Target

Integrin alpha 2 beta 1

Conjugation

Unconjugated

Tag

C-10xHis | Native

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human ITGA2 & ITGB1 Heterodimer Protein (Cat. No. IT1-H52W6) with a linear range of 0.078-2.5μg/mL (QC tested).