Phospho-Syk Antibody (pY525/526)
Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.
Product Specifications
Specifications
Western blot: 1:100-1:250, Immunohistochemistry (FFPE) : 1:50-1:100
UniProt
P43405
Host
Rabbit
Reactivity
Human
Immunogen
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Limitations
This phospho-Syk antibody is available for research use only.
Storage Conditions
Aliquot the phospho-Syk antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the phospho-Syk antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of lysate from human Raji cells that was untreated (-) and treated with 200 nm TPA for 30 min (+) .
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