NGFR Antibody

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.

Product Specifications

Specifications

Immunofluorescence: 1:100, Western blot: 1:500-1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

P08138

Host

Mouse

Reactivity

Human, Mouse

Immunogen

This NGFR antibody was produced from mouse immunized with NGFR recombinant protein.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

8G3G10

Applications

IF, WB, IHC, ELISA

Purity

Purified

Format

Purified

Limitations

This NGFR antibody is available for research use only.

Storage Conditions

Aliquot the NGFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the NGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Fluorescent confocal image of SY5Y cells stained with NGFR antibody. Alexa Fluor 488 secondary Ab (green) was used (1:1000, 1h) . Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min) .