CD133 Antibody / PROM1

CD133/PROM1/Prominin-1 may play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. [UniProt]

Product Specifications

Specifications

Western blot: 1:1000

UniProt

O43490

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant partial protein was used as the immunogen for this CD133 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

6H10-F1-C11

Applications

Purity

Protein G affinity

Format

Purified

Limitations

This CD133 antibody is available for research use only.

Storage Conditions

Store the CD133 antibody at -20°C.

Formulation

In PBS with 50% glycerol, 0.1 mg/mL BSA and 0.02% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CD133 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human CaCo2 cell lysate with CD133 antibody at 1:1000. Predicted molecular weight: ~97 kDa (unmodified), ~130 kDa (glycosylated) .