PCCA Antibody / Propionyl-CoA carboxylase alpha

The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Specifications

Specifications

Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:25

UniProt

P05165

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 362-390 from the human protein were used as the immunogen for the PCCA antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Limitations

This PCCA antibody is available for research use only.

Storage Conditions

Aliquot the PCCA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PCCA antibody may be required due to differences in protocols and secondary/substrate sensitivity.