PCCA Antibody / Propionyl-CoA carboxylase alpha
The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Specifications
Specifications
Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:25
UniProt
P05165
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A portion of amino acids 362-390 from the human protein were used as the immunogen for the PCCA antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC-P
Purity
Antigen affinity purified
Format
Purified
Limitations
This PCCA antibody is available for research use only.
Storage Conditions
Aliquot the PCCA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Image Legend
IHC testing of FFPE human heart tissue with PCCA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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