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CD326 Antibody

Mouse Monoclonal antibody againts EpCAM

Product Specifications

Product Name Alternative

EPCAM, GA733-2, EGP314, KSA, KS 1/4 antigen, Trop-1, M4S1, DIAR5, MIC18, TROP1

UniProt

P16422

Reactivity

Human

Immunogen

Small cell lung carcinoma cell line H69.

Target

CD326

Clonality

Monoclonal

Clone

VU-1D9

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 5-10 μg/ml; positive control: colon epithelium, antigen retrieval: heat (sodium citrate) + trypsin. Immunocytochemistry: Recommended dilution: 4 μg/ml. Western blotting: Recommended dilution: 1-4 μg/ml, non-reducing conditions. Flow cytometry: Recommended dilution: 1-3 μg/ml. Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate.

Tested Applications

FC, ICC, IHC-Fr, IHC-P, IP, WB

Preservative

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Isotype

Mouse IgG1

Entrez

4072

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