ATP5B Antibody
ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c) . It is the beta subunit of the catalytic core.
Product Specifications
Specifications
Western blot: 1:1000, Immunohistochemistry (FFPE) : 1:25-1:100, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25
UniProt
P06576
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 135-163 from the human protein were used as the immunogen for the ATP5B antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC-P, FACS, IF
Purity
Antigen affinity purified
Format
Purified
Limitations
This ATP5B antibody is available for research use only.
Storage Conditions
Aliquot the ATP5B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/atp5b-antibody-f54243
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic
Image Legend
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